Uracil Glycosylase Inhibitor (UGI)
- Uracil Glycosylase Inhibitor is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species
- Remains partially active following heat treatment at 95°C
- Can be used to prevent subsequent degradation of product DNA
The Uracil Glycosylase Inhibitor (UGI) of Bacillus subtilis bacteriophage PBS1 is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species. Inhibition of UDG occurs by reversible protein binding with a 1:1 UGD:UGI stoichiometry. UGI is capable of dissociating UDG-DNA complexes.
Product SourceAn E. coli strain that carries the cloned UGI gene from Bacillus subtilis bacteriophage PBS1.
The following reagents are supplied with this product:
Store at (°C) Concentration UDG Reaction Buffer -20 10 X
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