T7 Endonuclease I
Recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked ds-DNA.
- Recognition of mismatched DNA
- Resolve four-way junction or branched DNA
- Detection or cleavage of heteroduplex and nicked DNA
- Random cleavage of linear DNA for shotgun cloning
Quick Tips - Why don't my bands on a gel look correct after T7 digestion?
Quick Tips - Should I clean up my PCR before T7 endonuclease digestion?
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3.
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Recognizes non-perfectly matched DNA
Product SourceAn E. coli strain that carries a fusion of maltose binding protein and T7 Endonuclease I (T7 Endo I).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10 X
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