Home PCR, qPCR & Amplification Technologies Products Bsu DNA Polymerase, Large Fragment
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Catalog # Concentration Size List Price Your Price Quantity
M0330S 5,000 units/ml 200 units $74.00
M0330L 5,000 units/ml 1,000 units $293.00
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  • Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I (1), but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.

    Product Source

    An E. coli strain that contains a genetic fusion of the Bacillus subtilis DNA polymerase I gene (starting from codon 297 thus lacking the 5´→ 3´ exonuclease domain), and the gene coding for maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion is cleaved off in vitro. The remaining DNA polymerase is purified free of MBP.
    This product is related to the following categories:
    DNA Manipulation Products
    This product can be used in the following applications:
    Recombinase Polymerase Amplification and SIBA,
    Polymerases for DNA Manipulation,
    RT-PCR & cDNA Synthesis,
    Isothermal Amplification,
    PCR

    • Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

      Reaction Conditions

      1X NEBuffer™ 2
      Incubate at 37°C

      1X NEBuffer™ 2
      50 mM NaCl
      10 mM Tris-HCl
      10 mM MgCl2
      1 mM DTT
      (pH 7.9 @ 25°C)

      Heat Inactivation

      75°C for 20 minutes

      5' - 3' Exonuclease

      No

      3' - 5' Exonuclease

      No

      Strand Displacement

      +

      Unit Assay Conditions

      1X NEBuffer 2, 33 µM dNTPs including [3H]-dTTP and 70 µg/ml denatured herring sperm DNA.

    • Tools & Resources

    • Citations & Technical Literature

      • Citations

        Additional Citations

        • Hoser MJ, Mansukoski HK, Morrical SW, Eboigbodin KE (2014) Strand Invasion Based Amplification (SIBA®): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte PLoS One; 9(11), e112656. PubMedID: 25419812, DOI: 10.1371/journal.pone.0112656

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