Q5® High-Fidelity 2X Master Mix
cloned at NEB recombinant No hifi pcr 3

Fidelity at its finest – for over 10 years

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Master mix includes Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and hot start master mix formats (NEB #M0494)
Catalog # Concentration Size
M0492S 2 X 100 reactions (50 µl)
M0492L 2 X 500 reactions (50 µl)
Please enter a quantity for at least one size

Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
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