Q5U®  Hot Start High-Fidelity DNA Polymerase

Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

  • Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
  • Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
  • Generate higher assembly efficiency and improve accuracy in USER® cloning
  • Enable room temperature setup with aptamer-based hot start formulation
  • Utilize optimized protocols for recommended applications

 

 

 

Catalog # Concentration Size
M0515S 2,000 units/ml 100 units
M0515L 2,000 units/ml 500 units
Please enter a quantity for at least one size

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