New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

NEBNext® Q5U®  Master Mix

NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. 
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

  • Optimized for high yields in uracil-containing NGS library amplification
  • Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
Catalog # Concentration Size
M0597S 2 X 50 reactions
M0597L 2 X 250 reactions
Please enter a quantity for at least one size
Loading Spinner