NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.
Bulk packaging may also be available and requested for large recurring orders.
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The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
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