NEBNext® Q5U™ Master Mix

NEBNext^®Q5U^® Master Mix

NEBNext® Q5U™ Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

Optimized for high yields in uracil-containing NGS library amplification
Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
Minimizes GC bias, with superior performance across the GC spectrum
Ultra-high-fidelity amplification
Aptamer-based hot start for room-temperature reaction set-up without a separate activation step

Catalog #	Concentration	Size
M0597S	2 X	50 reactions
M0597L	2 X	250 reactions
Please enter a quantity for at least one size

Catalog #

Concentration

Size

M0597S

2 X

50 reactions

M0597L

2 X

250 reactions

Product Information
NEBNext Q5U Master Mix is optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.
The polymerase component of the master mix, Q5U, is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. The NEBNext Q5U Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.

NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

Product Categories:

Epigenetic Analysis,

Q5® High-Fidelity DNA Polymerases Products,

Epigenetics Products
,
Sample Prep for NGS & Target Enrichment Products
,
PCR, qPCR & Amplification Technologies Products

Applications:

Polymerases for NGS Library Preparation,

DNA Methylation Analysis,

Applications of Epigenetics
,
Methylated DNA Analysis Products
,
DNA Library Preparation
,
Sample Prep & Target Enrichment for NGS
,
Specialty PCR
- Properties & Usage
  
  Storage Temperature
  -20°C
Protocols, Manuals & Usage
- Protocols
  Reaction Setup NEBNext® Q5U™ Master Mix (NEB #M0597)
Tools & Resources
- Web Tools
  PCR Selector
FAQs & Troubleshooting
- FAQs
  Can NEBNext Q5U Master Mix be used to amplify templates containing uracils?
  
  Is the NEBNext Q5U Master Mix a hot start formulation?
  
  What cycling conditions should I use for the NEBNext Q5U Master Mix?
  
  How many cycles of PCR should I perform with the NEBNext Q5U Master Mix?
  
  Are the DNA products produced by the NEBNext Q5U Master Mix blunt-ended or do they have a single base 3´ overhang?
Citations & Technical Literature
- Feature Articles
  Enzymatic Methyl-seq: The Next Generation of Methylome Analysis
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0597S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0597G_v0_10043621
  
  M0597L_v0_10043619
  
  M0597S_v0_10043617
  
  M0597G_v0_10038705
  
  M0597S_v1_10046073
  
  M0597S_v1_10038707
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NEBNext^®Q5U^® Master Mix
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Licenses
  
  This product is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (not real-time) PCR in the research field only, but not real time PCR or digital PCR; (b) real-time PCR for use as a library preparation quantitation tool in Next Generation Sequencing workflows; (c) any in-vitro diagnostics applications, except for applications using real-time PCR or digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification, and the production of synthetic DNA.
  
  This product is licensed from University of Newcastle Upon Tyne under EPO Patent No. 1463809B1 and Australian Patent No. AU2003226543.
  
  Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based NEBNext Q5U Master Mix for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based NEBNext Q5U Master Mix requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.

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NEBNext^®Q5U^® Master Mix

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NEBNext® Q5U® Master Mix

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NEBNext^®Q5U^® Master Mix