NEBNext® Q5U®  Master Mix

NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. 
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

  • Optimized for high yields in uracil-containing NGS library amplification
  • Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
Catalog # Concentration Size
M0597S 2 X 50 reactions
M0597L 2 X 250 reactions
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