NEBNext® Q5U® Master Mix
NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.
- Optimized for high yields in uracil-containing NGS library amplification
- Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
- Minimizes GC bias, with superior performance across the GC spectrum
- Ultra-high-fidelity amplification
- Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
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Product Information
NEBNext Q5U Master Mix is optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.
The polymerase component of the master mix, Q5U, is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. The NEBNext Q5U Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration - This product is related to the following categories:
- Epigenetic Analysis,
- Q5® High-Fidelity DNA Polymerases Products,
- Epigenetics Products,
- PCR, qPCR & Amplification Technologies Products, NGS Sample Prep & Target Enrichment Products
- This product can be used in the following applications:
- Polymerases for NGS Library Preparation,
- DNA Methylation Analysis,
- Bisulfite Conversion,
- Epigenetics,
- DNA Library Preparation,
- Specialty PCR, NGS Sample Prep & Target Enrichment
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