EnGen® Spy Cas9 Nickase

icon_recomb icon_neb3_1 icon_incTemp37 icon_HI_65
  • Variant of Cas9 nuclease differing by a point mutation (D10A) in the RuvC nuclease domain
  • Capable of generating nicks, but not cleaving DNA
  • DNA double strand breaks can be generated, with reduced off-target cleavage, by targeting two sites with EnGen Cas9 Nickase in close proximity

Ordering Information

  • 1 μM
    70 pmol
  • 20 μM
    400 pmol
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