EnGen® Spy Cas9 Nickase
icon_recomb icon_neb3_1 icon_incTemp37 icon_HI_65

  • Variant of Cas9 nuclease differing by a point mutation (D10A) in the RuvC nuclease domain
  • Capable of generating nicks, but not cleaving DNA
  • DNA double strand breaks can be generated, with reduced off-target cleavage, by targeting two sites with EnGen Cas9 Nickase in close proximity
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Catalog # Concentration Size
M0650S 1 µM 70 pmol
M0650T 20 µM 400 pmol
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