EnGen® Spy dCas9 (SNAP-tag®)
- An inactive mutant of Cas9 nuclease that retains programmable DNA binding activity
- The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment
EnGen® Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity (1,2). EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag® allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
Product SourceEnGen® Spy dCas9 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 3.1 -20 10 X
- Product Categories:
- Genome Editing Products
- FAQs & Tech Tips
- Protocols & Manuals
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.