EnGen® Lba Cas12a (Cpf1)
EnGen Lba Cas12a (Cpf1) is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Targeting requires a gRNA complementary to the target site as well as a 5' TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends.
- T-rich TTTN PAM sequence opens up additional genomic regions for targeting
- Shorter, 40-44 base guide RNA
- Two nuclear localization signals for improved transport to the nucleus
- 5’ overhanging termini on cleavage products
- Active from 16 to 48°C
- Maintains activity at lower temperatures than the Acidaminococcus orthologs, permitting editing in ectothermic organisms such as zebra fish and xenopus
- High concentration liquid format can be used for microinjection, electroporation and lipofection
EnGen® Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) (1). Targeting requires a gRNA complementary to the target site as well as a 5’ TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3′ of the PAM and leaves 5′ overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
Product SourceEnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2.1 -20 10X
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