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Thermolabile USER® II Enzyme
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Thermolabile USER (Uracil-Specific Excision Reagent) II Enzyme generates a single nucleotide gap at the location of a uracil residue. It can be 100% inactivated at temperatures >65°C

Applications of Thermolabile USER II include:

  • Directional RNA Seq
  • NEBNext adaptor cleavage
  • USER cloning

Thermolabile USER II Enzyme

Mechanism
Antarctic Thermolabile Uracil DNA glycosylase (UDG) catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease III breaks the phosphodiester backbone at the 3´and 5´ sides of the abasic site. In addition to generating a different a 3´-terminus than USER Enzyme (a 3´-phospho-α, β-unsaturated aldehyde versus the 3´phosphate left by USER® Enzyme, NEB #M5505), Thermolabile USER II Enzyme (NEB #M5508) can also be completely heat inactivated after 10 minutes at 65°C.


Catalog # Concentration Size
M5508S 1,000 units/ml 50 units
M5508L 1,000 units/ml 250 units
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