DNA Library Prep for Illumina

The NEBNext® Ultra™ II solution for Illumina DNA library prep is a streamlined suite of reagents and workflows designed for the construction of high-quality libraries, using input amounts from pg to μg of DNA. Workflows are fast, user-friendly, and automatable. Available with or without an integrated enzymatic fragmentation step and optional PCR-free workflows, NEBNext offers flexible product formats maximal flexibility in your workflows. Designed for use in concert with NEBNext Multiplex Oligos for simple yet powerful NGS sample multiplexing. 
 
FEATURES ULTRA II DNA LIBRARY PREP ULTRA II FS DNA LIBRARY PREP
Unprecedented performance, enabling lower inputs and fewer PCR cycles Perform fragmentation, end repair, and dA-tailing with a single enzyme mix
ULTRA II INPUT AMOUNTS 500 pg – 1 µg of sheared DNA 100 pg – 500 ng of intact DNA
Available with or without beads?
Modules available?
ULTRA II PCR-FREE INPUT AMOUNTS 250 ng – 1,000 ng of sheared DNA 50 ng – 500 ng of intact DNA
Available with or without beads?
Modules available?
COMPATIBLE WITH FFPE DNA? Yes – for improved performance, consider the NEBNext FFPE DNA Repair Mix (NEB #M6630) Yes – for improved performance, consider the NEBNext FFPE DNA Repair Mix (NEB #M6630)
COMPATIBLE WITH METHYLOME ANALYSIS? Yes – Methylome analysis is supported; however, we recommend NEBNext EM-seq™ (NEB #E7120) No – not compatible due to potential for loss of methyl marks
COMPATIBLE WITH OXFORD NANOPORE TECHNOLOGIES? Yes. We recommend the NEBNext Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7810).

 
NEBNext DNA library prep reagents are also available at scale for your high-throughput and/or development needs. Contact the Customized Solutions Team for more details on custom product options, large volumes, or OEM materials.  
 

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FAQs for DNA Library Prep for Illumina
Ultra II FS DNA Library Preparation Workflow
ChIP DNA Library Preparation Workflow for Illumina
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Ultra II DNA Library Preparation for Illumina
DNA Product Details for Illumina
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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