α2-3,6,8 Neuraminidase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0720S     -20       α2-3,6,8 Neuraminidase P0720SVIAL -20 1 x 0.04 ml 50,000 units/ml   GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X
P0720L     -20       α2-3,6,8 Neuraminidase P0720LVIAL -20 1 x 0.2 ml 50,000 units/ml   GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X

Companion Products

• O-Glycosidase & Neuraminidase Bundle
• PNGase F
• PNGase F (Glycerol-free)
• O-Glycosidase

1. Roggentin, P. et al. (1988). FEBS Lett. 238(1), 31-34.
2. Guan, C., New England Biolabs unpublished observations.
3. Wong-Madden, S.T. and Landry, D. (1995). Glycobiology. 5, 19-28.

1. Typical Reaction Conditions for α2-3,6,8 Neuraminidase (P0720)
2. Endo-α-N-Acetylgalactosaminidase Application Note 1

• Glycobiology Unit Conversion Chart

1. Can I use α2-3,6,8 Neuraminidase in a double digest with Endo H(H_f) or PNGase F?
2. Is α2-3,6,8 Neuraminidase active at higher pH levels?
3. How much exoglycosidase should be used?
4. Do detergents inhibit exoglycosidases/endoglycosidases?
5. What is a good positive control for α2-3,6,8 Neuraminidase?
6. Why have the NEB Glycosidase enzymes changed reaction buffers? What are the new reaction buffers and can I still use an enzyme with its old buffer? Where can I find the composition of the old buffers?
7. What are glycosidases and their uses?
8. Is it necessary to treat my glycoprotein concomitantly with Neuraminidase and O-Glycosidase?
9. Do the NEB Neuraminidase enzymes cleave both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) residues?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide

• Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0720S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0720S_L_v1_0161603
• P0720S_L_v1_0171609
• P0720S_L_v1_0171612
• P0720S_L_v1_0191707
• P0720S_L_v1_0201710
• P0720S_L_v1_0201801
• P0720L_v1_10010266
• P0720L_v1_10015145
• P0720S_v1_10018751
• P0720S_v1_10026475
• P0720L_v1_10026474
• P0720S_v1_10031657
• P0720L_v1_10044263
• P0720S_v1_10044262
• P0720S_v1_10044661
• P0720L_v1_10054879
• P0720L_v1_10056317
• P0720L_v1_10061039
• P0720S_v1_10062665
• P0720L_v1_10065575
• P0720S_v1_10065577
• P0720L_v1_10075882
• P0720S_v1_10102998
• P0720S_v1_10113722
• P0720L_v1_10103000
• P0720S_v1_10117868
• P0720L_v1_10133654
• P0720L_v1_10152115
• P0720S_v1_10156498
• P0720L_v1_10169875
• P0720S_v1_10174000
• P0720L_v1_10178400

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• α2-3,6,8 Neuraminidase
• GlycoBuffer 1

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.