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PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR.

PCR, Polymerases & Amplification Technologies

Luna® Universal One-Step RT-qPCR Kit

Luna® Universal Probe One-Step RT-qPCR Kit

Luna® Universal Probe qPCR Master Mix

Luna® Universal qPCR Master Mix

Q5® High-Fidelity DNA Polymerases

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Ultra™ II Q5® Master Mix

Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity PCR Kit

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity DNA Polymerase

Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

OneTaq® 2X Master Mix with GC Buffer

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase

OneTaq® GC Reaction Buffer Pack

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start DNA Polymerase

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Standard Reaction Buffer Pack

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

EpiMark® Hot Start Taq DNA Polymerase

Hot Start Taq 2X Master Mix

Hot Start Taq DNA Polymerase

Multiplex PCR 5X Master Mix

OneTaq® 2X Master Mix with GC Buffer

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase

OneTaq® GC Reaction Buffer Pack

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start DNA Polymerase

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Standard Reaction Buffer Pack

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Quick-Load® Taq 2X Master Mix

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Taq 2X Master Mix

Taq 5X Master Mix

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Taq PCR Kit

ThermoPol® II (Mg-free) Reaction Buffer Pack

ThermoPol® Reaction Buffer Pack

LongAmp® DNA Polymerases

LongAmp® Hot Start Taq 2X Master Mix

LongAmp® Hot Start Taq DNA Polymerase

LongAmp® Taq 2X Master Mix

LongAmp® Taq DNA Polymerase

LongAmp® Taq PCR Kit

LongAmp® Taq Reaction Buffer Pack

Luna® qPCR & RT-qPCR Products

Luna® Universal One-Step RT-qPCR Kit

Luna® Universal Probe One-Step RT-qPCR Kit

Luna® Universal Probe qPCR Master Mix

Luna® Universal qPCR Master Mix

Other PCR Polymerases

Deep Vent® (exo-) DNA Polymerase

Hemo KlenTaq®

Vent® (exo-) DNA Polymerase

Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

Phusion® GC Buffer Pack

Phusion® HF Buffer Pack

Phusion® High-Fidelity DNA Polymerase

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Phusion® Hot Start Flex 2X Master Mix

Phusion® Hot Start Flex DNA Polymerase

Phusion® High-Fidelity PCR Kit

Master Mixes

Hot Start Taq 2X Master Mix

LongAmp® Hot Start Taq 2X Master Mix

LongAmp® Taq 2X Master Mix

Multiplex PCR 5X Master Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

OneTaq® 2X Master Mix with GC Buffer

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Phusion® Hot Start Flex 2X Master Mix

Q5® High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity 2X Master Mix

Quick-Load® Taq 2X Master Mix

Taq 2X Master Mix

Taq 5X Master Mix

DNA Manipulation

Bsu DNA Polymerase, Large Fragment

DNA Polymerase I (E. coli)

DNA Polymerase I, Large (Klenow) Fragment

Klenow Fragment (3'→5' exo-)

phi29 DNA Polymerase Reaction Buffer

PreCR® Repair Mix

Quick Blunting™ Kit

Sulfolobus DNA Polymerase IV

T4 DNA Polymerase

T7 DNA Polymerase (unmodified)

Terminal Transferase

Isothermal Amplification & Strand Displacement

Bst 2.0 DNA Polymerase

Bst 2.0 WarmStart® DNA Polymerase

Bst 3.0 DNA Polymerase

Bst DNA Polymerase, Full Length

Bst DNA Polymerase, Large Fragment

IsoAmp® II Universal tHDA Kit

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

Klenow Fragment (3'→5' exo-)

phi29 DNA Polymerase

PicoPLEX™ WGA Kit

WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

WarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

3´-Biotin-GTP

3´-Desthiobiotin-GTP

5-methyl-dCTP

7-deaza-dGTP

Acyclonucleotide Set

Adenosine 5'-Triphosphate (ATP)

dATP Solution

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

Ribonucleotide Solution Mix

Ribonucleotide Solution Set

cDNA Synthesis & Reverse Transcriptases

AMV First Strand cDNA Synthesis Kit

AMV Reverse Transcriptase

DNA Polymerase I (E. coli)

E. coli Poly(A) Polymerase

M13KO7 Helper Phage

M-MuLV Reverse Transcriptase

M-MuLV Reverse Transcriptase Reaction Buffer

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Oligo d(T)18 mRNA Primer

Oligo d(T)23 VN

OneTaq® One-Step RT-PCR Kit

OneTaq® RT-PCR Kit

Poly(U) Polymerase

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

Random Primer 9

RNase H

RNase Inhibitor, Murine

SP6 RNA Polymerase

T3 RNA Polymerase

T4 RNA Ligase 2, truncated KQ

WarmStart® RTx Reverse Transcriptase

RT-PCR

AMV First Strand cDNA Synthesis Kit

AMV Reverse Transcriptase

M-MuLV Reverse Transcriptase

M-MuLV Reverse Transcriptase Reaction Buffer

Oligo d(T)18 mRNA Primer

Oligo d(T)23 VN

OneTaq® One-Step RT-PCR Kit

OneTaq® RT-PCR Kit

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

Random Primer 9

T4 RNA Ligase 2, truncated KQ

WarmStart® RTx Reverse Transcriptase

Reaction Buffers

Hemo KlenTaq® Reaction Buffer Pack

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

LongAmp® Taq Reaction Buffer Pack

Magnesium Chloride (MgCl2) Solution

Magnesium Sulfate (MgSO4) Solution

M-MuLV Reverse Transcriptase Reaction Buffer

OneTaq® GC Reaction Buffer Pack

OneTaq® Standard Reaction Buffer Pack

phi29 DNA Polymerase Reaction Buffer

Phusion® GC Buffer Pack

Phusion® HF Buffer Pack

Q5® Reaction Buffer Pack

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

ThermoPol® Reaction Buffer Pack

β-Nicotinamide adenine dinucleotide (NAD+)

Q5® High-Fidelity DNA Polymerases

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Ultra™ II Q5® Master Mix

Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity PCR Kit

Q5® Hot Start High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity DNA Polymerase

Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

OneTaq® 2X Master Mix with GC Buffer

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase

OneTaq® GC Reaction Buffer Pack

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start DNA Polymerase

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Standard Reaction Buffer Pack

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

EpiMark® Hot Start Taq DNA Polymerase

Hot Start Taq 2X Master Mix

Hot Start Taq DNA Polymerase

Multiplex PCR 5X Master Mix

OneTaq® 2X Master Mix with GC Buffer

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase

OneTaq® GC Reaction Buffer Pack

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start DNA Polymerase

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Standard Reaction Buffer Pack

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Quick-Load® Taq 2X Master Mix

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Taq 2X Master Mix

Taq 5X Master Mix

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Taq PCR Kit

ThermoPol® II (Mg-free) Reaction Buffer Pack

ThermoPol® Reaction Buffer Pack

LongAmp® DNA Polymerases

LongAmp® Hot Start Taq 2X Master Mix

LongAmp® Hot Start Taq DNA Polymerase

LongAmp® Taq 2X Master Mix

LongAmp® Taq DNA Polymerase

LongAmp® Taq PCR Kit

LongAmp® Taq Reaction Buffer Pack

Luna® qPCR & RT-qPCR Products

Luna® Universal One-Step RT-qPCR Kit

Luna® Universal Probe One-Step RT-qPCR Kit

Luna® Universal Probe qPCR Master Mix

Luna® Universal qPCR Master Mix

Other PCR Polymerases

Deep Vent® (exo-) DNA Polymerase

Hemo KlenTaq®

Vent® (exo-) DNA Polymerase

Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

Phusion® GC Buffer Pack

Phusion® HF Buffer Pack

Phusion® High-Fidelity DNA Polymerase

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Phusion® Hot Start Flex 2X Master Mix

Phusion® Hot Start Flex DNA Polymerase

Phusion® High-Fidelity PCR Kit

Master Mixes

Hot Start Taq 2X Master Mix

LongAmp® Hot Start Taq 2X Master Mix

LongAmp® Taq 2X Master Mix

Multiplex PCR 5X Master Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

OneTaq® 2X Master Mix with GC Buffer

OneTaq® 2X Master Mix with Standard Buffer

OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with Standard Buffer

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Quick-Load® 2X Master Mix with GC Buffer

OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Phusion® Hot Start Flex 2X Master Mix

Q5® High-Fidelity 2X Master Mix

Q5® Hot Start High-Fidelity 2X Master Mix

Quick-Load® Taq 2X Master Mix

Taq 2X Master Mix

Taq 5X Master Mix

DNA Manipulation

Bsu DNA Polymerase, Large Fragment

DNA Polymerase I (E. coli)

DNA Polymerase I, Large (Klenow) Fragment

Klenow Fragment (3'→5' exo-)

phi29 DNA Polymerase Reaction Buffer

PreCR® Repair Mix

Quick Blunting™ Kit

Sulfolobus DNA Polymerase IV

T4 DNA Polymerase

T7 DNA Polymerase (unmodified)

Terminal Transferase

Isothermal Amplification & Strand Displacement

Bst 2.0 DNA Polymerase

Bst 2.0 WarmStart® DNA Polymerase

Bst 3.0 DNA Polymerase

Bst DNA Polymerase, Full Length

Bst DNA Polymerase, Large Fragment

IsoAmp® II Universal tHDA Kit

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

Klenow Fragment (3'→5' exo-)

phi29 DNA Polymerase

PicoPLEX™ WGA Kit

WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

WarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

3´-Biotin-GTP

3´-Desthiobiotin-GTP

5-methyl-dCTP

7-deaza-dGTP

Acyclonucleotide Set

Adenosine 5'-Triphosphate (ATP)

dATP Solution

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

Ribonucleotide Solution Mix

Ribonucleotide Solution Set

cDNA Synthesis & Reverse Transcriptases

AMV First Strand cDNA Synthesis Kit

AMV Reverse Transcriptase

DNA Polymerase I (E. coli)

E. coli Poly(A) Polymerase

M13KO7 Helper Phage

M-MuLV Reverse Transcriptase

M-MuLV Reverse Transcriptase Reaction Buffer

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Oligo d(T)18 mRNA Primer

Oligo d(T)23 VN

OneTaq® One-Step RT-PCR Kit

OneTaq® RT-PCR Kit

Poly(U) Polymerase

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

Random Primer 9

RNase H

RNase Inhibitor, Murine

SP6 RNA Polymerase

T3 RNA Polymerase

T4 RNA Ligase 2, truncated KQ

WarmStart® RTx Reverse Transcriptase

RT-PCR

AMV First Strand cDNA Synthesis Kit

AMV Reverse Transcriptase

M-MuLV Reverse Transcriptase

M-MuLV Reverse Transcriptase Reaction Buffer

Oligo d(T)18 mRNA Primer

Oligo d(T)23 VN

OneTaq® One-Step RT-PCR Kit

OneTaq® RT-PCR Kit

ProtoScript® First Strand cDNA Synthesis Kit

ProtoScript® II First Strand cDNA Synthesis Kit

ProtoScript® II Reverse Transcriptase

Random Primer 9

T4 RNA Ligase 2, truncated KQ

WarmStart® RTx Reverse Transcriptase

Reaction Buffers

Hemo KlenTaq® Reaction Buffer Pack

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

LongAmp® Taq Reaction Buffer Pack

Magnesium Chloride (MgCl2) Solution

Magnesium Sulfate (MgSO4) Solution

M-MuLV Reverse Transcriptase Reaction Buffer

OneTaq® GC Reaction Buffer Pack

OneTaq® Standard Reaction Buffer Pack

phi29 DNA Polymerase Reaction Buffer

Phusion® GC Buffer Pack

Phusion® HF Buffer Pack

Q5® Reaction Buffer Pack

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

ThermoPol® II (Mg-free) Reaction Buffer Pack

ThermoPol® Reaction Buffer Pack

β-Nicotinamide adenine dinucleotide (NAD+)