cloned at NEB recombinant engineered neb31 dil_A 55 80 Heat

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at

NEB restriction endonuclease that recognizes the sequence CAGTG_NN^


  • Sourced from an E. coli strain that carries the cloned and modified BtsI gene from Bacillus thermoglucosidasius
  • 100% activity in rCutSmart™ Buffer allowing for easier double digests
  • Not sensitive to dam, dcm or mammalian CpG methylation
  • Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Cut Site: CAGTG(2/0)


Catalog # Concentration Size
R0664S 1,000 units/ml 100 units
Please enter a quantity for at least one size
Loading Spinner