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SNAP-Surface® Alexa Fluor® 488

SNAP-Surface® Alexa Fluor® 488 is a photostable green fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells.

  • This is a cell impermeable substrate based on BG-Alexa Fluor 488
  • It is suitable for standard fluorescein filter sets
  • It has an excitation maximum at 496 nm and emission maximum at 520 nm
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Catalog # Concentration Size
S9129S Not Applicable 50 nmol
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  • Product Information
    This package includes 50 nmol of SNAP-Surface Alexa Fluor 488 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag
    substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.
    Figure 1: Live HEK293 cells transiently transfected with pSNAP-ADRβ2 and pSNAP-H2B

    Figure 1: Live HEK293 cells transiently transfected with pSNAP-ADRβ2 and pSNAP-H2B

    Cells were sequentially labeled with SNAP-Surface Alexa Fluor 488 (green) and SNAP-Cell TMR-Star (red) for 15-30 minutes.
    Figure 2: Excitation (dotted line) and emission (full line) spectra of SNAP-Surface Alexa Fluor® 488 coupled to SNAP-tag in buffer at pH 7.5
    This product is related to the following categories:
    SNAP-tag Substrates Products ,
    Protein Labeling Products
    This product can be used in the following applications:
    Snap Surface Products,
    Pulse Chase,
    In vivo Imaging,
    Receptor Internalization,
    Protein Localization,
    Protein Labeling Snap Clip
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