Monarch^® RNA Cleanup Wash Buffer

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T2042L     25       Monarch® RNA Cleanup Wash Buffer T2042-2 25 2 x 20 ml Not Applicable

1. Purification of RNA from the Aqueous Phase Following TRIzol^®/Chloroform Extraction using the Monarch^® RNA Cleanup Kits
2. Separation of Large and Small RNA into Fractions using the Monarch^® RNA Cleanup Kits
3. Extraction of RNA from Agarose Gels using the Monarch^® RNA Cleanup Kits
4. Monarch^® RNA Cleanup Kit Protocol
5. RNA Extraction from Cells Using the Monarch RNA Cleanup Kits
6. RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch RNA Cleanup Kits
7. RNA Extraction from Saliva Using the Monarch RNA Cleanup Kits
8. Automation of Viral RNA Extraction from Saliva Using the Monarch RNA Cleanup Kit

• Avoiding Ribonuclease Contamination
• Guidelines for RNA Quantitation
• Guidelines for Working with RNA During RNA Cleanup

1. What is the composition of each buffer provided with the Monarch RNA Cleanup Kits?
2. What factors affect my (A260/A230) when using the Monarch RNA Cleanup Kits?
3. Can I purchase Monarch buffers and columns separately?
4. Can extraction of RNA with the Monarch kits be automated?
5. Can the Monarch RNA Cleanup Kits be used for RNA extraction?

• Troubleshooting Guide for RNA Cleanup

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T2042L_v1
• T2042L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2042L_v1_0011806
• T2042L_v1_10041699
• T2042L_v1_10059917
• T2042L_v1_10068604
• T2042L_v1_10099687
• T2042L_v2_10109334

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® RNA Cleanup Wash Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.