A Typical Deadenylation Reaction (M0331)

Combine the following components in sterile microfuge tube:

10X NEBuffer 1 2 μl
5–50 pmol 5´-adenylated DNA or RNA variable
H2O variable
5´ Deadenylase (50 units/μl) 1 μl
Total reaction 20 μl

Incubate at 30°C for 0.5–1 hour