Standard E. coli DNA Gyrase Reaction (M0306)

Overview

Mix the following components in an eppendorf tube as follows:

5X DNA Gyrase reaction buffer: 6ul
DNA Gyrase (M0306): 1ul
DNA (relax, circular): 500ng-2.5ug
H2O: to a total of 30ul
Incubate the mixture for 30min @37°C