NEBNext End Repair cDNA Library (E6114)

Protocol

Mix the following components in a sterile microfuge tube: 

Purified double stranded cDNA:   50 μl 
NEBNext End Repair Reaction Buffer (10X):   20 μl 
NEBNext End Repair Enzyme Mix:   10 μl 
Nuclease-Free Water:   120 μl 
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total volume:   200 μl

Incubate in a thermal cycler for 30 minutes at 20°C.

Purify DNA sample on one column.