Home Protocols Protocol for Tma Endonuclease III (M0291)

Protocol for Tma Endonuclease III (M0291)

1) Reaction set-up:

DNA 5 pmol
ThermoPol® Reaction Buffer (NEB #B9004) (10X) 1-5 µl
Tma Endonuclease III (NEB #M0291) 1 µl (10 units)
H2O Up to 10-50 µl

2) Incubate at 65°C for 1 hour.