Protocol for cleavage with McrBC (M0272)

Reaction Conditions:


1X NEBuffer 2, supplemented with 100 µg/ml BSA, 1 mM GTP. Incubate at 37°C.

Standard Protocol:

 

  1. Set up the following reaction on ice.

    COMPONENT  50 µl REACTION 
    NEBuffer 2 (10X) 
    5 µl (1X) 
    BSA (100X) 
     0.5 µl 
    GTP (100X) 
    0.5 µl 
    Methylated DNA
    1 µg
    McrBC 
    1 µl  (10 units)
     dH20  up to 50 μl
     

  2. Incubate at 37°C for 1-2 hours.

  3. Inactivate at 65°C for 20 minutes.

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Related Products:
McrBC