NEB scientists are often involved in the development of online tools that will aid in their research. We are now making these tools, and, in some circumstances, the source code, available for you to evaluate. These tools are not yet optimized for design and usability, so we are looking for your feedback on their functionality and utility to help us improve them for future use.
Ligase fidelity tools (for the design of high-fidelity Golden Gate Assemblies)
- Ligase Fidelity Viewer™ (v2)
Visualize overhang ligation preferences
Predict high-fidelity junction sets
Split DNA sequence for scarless high-fidelity assembly
Restriction enzyme tools
Virtually digest a DNA sequence with each of the known Type II restriction enzymes
- Genomes and Restriction Enzyme Frequencies
A resource detailing restriction enzyme frequencies (number of cuts) in various genomes
- Glycan Analyzer Code
Glycan Analyzer automates the structural annotation of UPLC-FLR-MS N-glycan profiles obtained following digestion with one or more exoglycosidases. We are making the code behind the tool available to allow its utility to be further developed by the glycobiology research community.
- Primer Monitor
Evaluate the potential impact of different SARS-CoV-2 variants on commonly-used and user-defined primer sets.
- Tm API
A public Tm API allowing programmatic access to the NEB Tm calculator for incorporation in automated high throughput primer design workflows.
NGS Sample Prep & Target Enrichment
- NEBNext Index Oligo Selector
This tool assists users in selecting barcode combinations when pooling NEBNext® Multiplex Oligos for Illumina®.
Please share your thoughts on how these tools and/or their code are working for you. Additionally, if you have any suggestions for new online tools that would help your research, please let us know. View NEB’s full list of online tools and mobile apps.Share Feedback
These software tools, and their underlying code, are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its software tools for various applications, the use of this software tool may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please email us at [email protected].
Use Restriction; The tools are for internal research only. No commercial use, reproduction or distribution of the tools is permitted. You shall not cause or allow the reverse engineering, disassembly, or decompilation of a tool.
Disclaimer of Liability; Limitation of Liability: THE TOOLS ARE EXPERIMENTAL IN NATURE AND PROVIDED “AS IS” WITHOUT ANY EXPRESS OR IMPLIED WARRANTY OF ANY KIND. IF YOU USE A TOOL, YOU, ON BEHALF OF THE COMPANY OR INSTITUTION YOU REPRESENT, ASSUME THE ENTIRE RISK REGARDING USES, OUTPUTS, ANALYSIS, RESULTS AND PERFORMANCE OF THE TOOL.
Feedback. Any feedback you provide regarding your use of a tool may be used by NEB for any purpose.
Learn more about NEB’s commitment to basic research
Advances in understanding ligase fidelity, bias, and efficiency in Golden Gate Assembly allows for assembly of more than 50 fragments in a single reaction.