NEB Diluent and Buffer Table

Composition List for NEB Diluents and Buffers

You can find the composition of NEB buffers and diluents in the table below. The columns represent the diluent or buffer referenced, and the rows represent the individual diluent or buffer components. The squares within the table indicate the final concentration of components for a 1X concentration of the buffer or diluent defined by the column. The final row of the table indicates the final pH of the mixture.

We are excited to announce that we all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Components T4 DNA Ligase Reaction Buffer | B0202 StickTogether™ DNA Ligase Buffer | B0535 ThermoPol® Reaction Buffer Pack | B9004 T4 Polynucleotide Kinase Reaction Buffer | B0201 NEBuffer™ EcoRI/SspI | B0101 Antarctic Phosphatase Reaction Buffer | B0289 Exonuclease I Reaction Buffer | B0293 Exonuclease VII Reaction Buffer | B0379 Diluent A | B8001 Diluent B | B8002 Diluent C | B8003 NEBuffer™ r1.1 | B6001 NEBuffer™ r2.1 | B6002 rCutSmart™ Buffer | B6004
Tris-HCl 50 mM 66 mM 20 mM 70 mM 100 mM 50 mM 10 mM 10 mM 10 mM 10 mM
Bis-Tris-Propane-HCl 50 mM 10 mM
Tris-acetate 20 mM
Sodium Phosphate 50 mM
Potassium Acetate 50 mM
Magnesium Acetate 10 mM
EDTA 8 mM 0.1 mM 0.1 mM 0.1 mM
NaCl 50 mM 300 mM 250 mM 50 mM
KCl 10 mM 50 mM
MgCl2 10 mM 10 mM 10 mM 10 mM 1 mM 6.7 mM 10 mM 10 mM
ZnCl2 0.1 mM
MgSO4 2 mM
(NH4)2SO4 10 mM
Glycine-KOH 67 mM
BSA 200 µg/ml 500 µg/ml 200 µg/ml
Recombinant Albumin 100 µg/ml 100 µg/ml 100 µg/ml
ATP 1 mM 1 mM
DTT 10 mM 1 mM 5 mM 1 mM 1 mM 1 mM
β-ME 10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000) 7.5%
Triton® X-100 0.1% 0.025% 0.15%
Glycerol 50% 50% 50%
pH @ 25 7.50 7.60 8.80 7.60 7.50 6.00 9.50 8.00 7.40 7.40 7.40 7.00 7.90 7.90
Loading Spinner