Fourth of July Shipping Notice: Orders placed on Friday, July 1, 2022, will be shipped on Tuesday, July 5, 2022, for delivery on Wednesday, July 6, 2022.

DNA Ligase Selection Chart

While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. To select a ligase based upon the type of substrate present, please visit Substrate-based Ligase Selection Chart.  In addition, the Properties of DNA and RNA Ligases will help you understand the differences between them.


Blunt/TA
Ligase
Master Mix
Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Hi-T4™ DNA Ligase   Immobilized T4 DNA Ligase Salt-T4® DNA Ligase  Quick
Ligation™
Kit
NEBridge® Ligase Master Mix  T3 DNA
Ligase
T7 DNA
Ligase
HiFi Taq DNA Ligase
Taq DNA
Ligase
9°N™ DNA
Ligase
NEBNext Quick Ligation™ Module SplintR® Ligase  E.coli DNA Ligase
DNA APPLICATIONS
Ligation of sticky ends ★★ ★★★ ★★ ★★  ★★ ★★★  ★★
★★★ ★★ ★★ ★ 
   ★
Ligation of blunt ends ★★★ ★★ ★★  ★★   ★★
★★★   ★★
 


   
T/A cloning ★★★ ★★ ★★  ★★   ★★
★★    


   
Electroporation

★★★ ★★  ★★  

 

 


   
Golden Gate Assembly       ★★★  a       ★★★                
Ligation of sticky ends only



     
 
★★★  


   
Repair of nicks in dsDNA ★★ ★★ ★★ ★★★  ★★★   ★★★
★★   ★★ ★★ ★★  ★★ ★★
★★ ★★ 
High-complexity library cloning ★★ ★★ ★★ ★★★       ★★  

 


   
Adapter Ligation  ▲
★★★ ★★ ★★       ★★  
 

   
Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)



     
 

 ★★★ ★★ ★★
   
Ligation-Dependent RNA Sequence & SNP Detection


     
 

 


★★★  
Ligation of adjacent ssDNAs on an RNA splint



     
 

     
 ★★★  
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)

     
 
 

   
FEATURES
Salt tolerance
(>2X that for T4 DNA Ligase)




     
 
 


   
Ligation in 15 min. or less
       
 ✓  
Master Mix Formulation

     
 

 

   
Thermostable



     
 

 ✓
   
Thermotolerant



   
 

 ✓
   
Recombinant  ✓         ✓
Reusable/Removable



      
 






 
KEY
★★★ Optimal, recommended ligase for selected application
★★ Works well for selected application
Will perform selected application, but is not recommended
Please consult the specific NGS protocol to determine the optimal enzyme for your needs
 a Not yet tested 
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