Protease Selection Chart

NEB offers several proteases with different recognition sites.

Product NEB # Recognition Site
Factor Xa Protease P8010 Ile-Glu/Asp-Gly-Arg
Enterokinase (enteropeptidase), light chain P8070 Asp-Asp-Asp-Asp-Lys
Furin P8077 Arg-X-X-Arg
Proteinase K P8107 non-specific, subtilisin-related serine protease
Thermolabile Proteinase K P8111 non-specific, subtilisin-related serine protease
Trypsin-ultra™, Mass Spectrometry Grade P8101 Lys/Arg-X-X-X
α-Lytic Protease P8113 XX-T/A/S/VXX
Endoproteinase GluC P8100 Glu-X-X-X or Asp-X-X-X
Endoproteinase AspN P8104 X-X-X-Asp
Endoproteinase LysC P8109 X-X-Lys-X-X-X
TEV Protease P8112 ENLYFQ▼(S/G/A/M/C/H)