NEB® offers a large selection of fluorescent labels (substrates) for SNAP- and CLIP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker, while CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. These substrates will label their respective tags without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell® and CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface® and CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.
|Substrates||NEB #||Excitation*||Emission* ✝||Size|
|SNAP-Surface® 488||#S9124||506||526||50.0 nmol|
|SNAP-Surface® 649||#S9159||655||676||50.0 nmol|
|SNAP-Surface® 594||#S9134||606||626||50.0 nmol|
|SNAP-Surface® 549||#S9112||560||575||50.0 nmol|
|SNAP-Surface® Alexa Fluor® 647||#S9136||652||670||50.0 nmol|
|SNAP-Surface® Alexa Fluor® 488||#S9129||496||520||50.0 nmol|
|SNAP-Surface® Alexa Fluor® 546||#S9132||558||574||50.0 nmol|
* Excitation and emission values determined experimentally for labeled protein tag.
† Colors are based on the electromagnetic spectrum. Actual color visualization may vary.
SNAP-tag®, SNAP-Cell®, SNAP-Surface® and NEB® are registered tradmarkes of New England Biolabs, Inc.
CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.