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Thermophilic DNA Polymerases

NEB supplies many thermophilic DNA polymerases; several characteristics should be considered when choosing the one best suited to your particular research needs.


Vent® Vent® (exo-) Deep Vent® Deep Vent® (exo-) Taq 9°N™ Phusion®** Long Amp® Taq Long Amp® Hot Start Taq One Taq® Q5™
Exonuclease Activities
proofreading exonuclease yes no yes no no no* yes yes yes yes yes
5'—>3' exonuclease activity no no no no yes no no yes yes yes no
Thermal Stability
Half life at 95°C 6.7 hours 6.7 hours 23 hours 23 hours 0.9 hours 6.7 hours >6 hours at 96°C ND ND ND ND
Half life at 100°C 1.8 hours 1.8 hours 8 hours 8 hours <0.1 hours <1.8 hours 2 hours at 98°C ND ND ND ND
Primer Extension Characteristics
Units/100 µl Primer Extension Rxn 0.4-2 units 1-4units 0.4-2 units 1-4 units 2.5-5 units 1-2 units 1-2 units 2-10 units 2-10 units 2.5-5 units 1-2 units
Fidelity to Taq 5X 2X 6X 2X 1X ND >50X 2X 2X 2X >100X
Resulting DNA Ends blunt 3´ A blunt 3´ A 3´ A 3´ A blunt blunt 3´ A
blunt
3´ A
blunt
3´ A
blunt
blunt
Strand Displacement yes; temp. dependent yes; temp. dependent yes; temp. dependent yes; temp. dependent no yes no yes; temp. dependent yes; temp. dependent yes; temp. dependent no
Longest Primer Extension to Date 13.2 kb 15.0 kb 14.0 kb 15.0 kb 8.1 kb 37 kb 40 kb 40 kb 15.0 kb 20 kb 20 kb

Vent®, LongAmp® and OneTaq® are registered trademarks of New England Biolabs, Inc.
Deep Vent®, 9°N™ and Q5™ are trademarks of New England Biolabs, Inc.

* 1-5% of wild type exonuclease activity is present in the modified enzyme.

** Notice to Customers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. Under agreement with, and under the performance specifications of Thermo Fisher Scientific.

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