Time-Saver™ Qualified Enzymes

Recombinant Digest in 5 minutes
Enzymes that are Time-Saver qualified will digest unit assay substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions Digest in 15 minutes
Not completely digested in 15 minutes
NT Not tested.

Enzyme Sequence Recombinant Time-Saver Qualified Unit Assay Substrate Plasmid Substrate PCR Substrate
AatII § GACGT/C    
Acc65I § G/GTACC    
AccI § GT/MKAC    
AciI § CCGC(-3/-1)    
AclI § AA/CGTT    
AcuI § CTGAAG(16/14)    
AflII § C/TTAAG    
AgeI-HF® § A/CCGGT    
AhdI § GACNNN/NNGTC    
AluI § AG/CT    
AlwNI § CAGNNN/CTG    
ApaI § GGGCC/C    
ApaLI § G/TGCAC    
ApeKI § G/CWGC    
ApoI § R/AATTY    
ApoI-HF § R/AATTY    
AscI § GG/CGCGCC     NT
AseI § AT/TAAT     NT
AvaI § C/YCGRG    
AvaII § G/GWCC    
AvrII § C/CTAGG     NT NT
BaeGI § GKGCM/C    
BaeI § (10/15)ACNNNNGTAYC(12/7)    
BamHI § G/GATCC    
BamHI-HF® § G/GATCC    
BbsI § GAAGAC(2/6)    
BbsI-HF® § GAAGAC(2/6)    
BbvI § GCAGC(8/12)    
BciVI § GTATCC(6/5)    
BclI § T/GATCA    
BclI-HF § T/GATCA    
BcoDI § GTCTC(1/5)    
BfuAI § ACCTGC(4/8)    
BglI § GCCNNNN/NGGC    
BglII § A/GATCT    
BlpI § GC/TNAGC    
BmgBI § CACGTC(-3/-3)    
BmtI-HF® § GCTAG/C    
BpuEI § CTTGAG(16/14)    
BsaAI § YAC/GTR    
BsaHI § GR/CGYC    
BsaI-HF®v2 § GGTCTC(1/5)    
BsaWI § W/CCGGW    
BsaXI § (9/12)ACNNNNNCTCC(10/7)  
BseRI § GAGGAG(10/8)    
BsgI § GTGCAG(16/14)    
BsiEI § CGRY/CG    
BsiHKAI § GWGCW/C    
BsiWI § C/GTACG    
BsiWI-HF® § C/GTACG    
BslI § CCNNNNN/NNGG    
BsmAI § GTCTC(1/5)    
BsmBI-v2 § CGTCTC    
BsmI § GAATGC(1/-1)    
BsoBI § C/YCGRG    
BspCNI § CTCAG(9/7)    
BspEI § T/CCGGA    
BspHI § T/CATGA    
Bsp1286I § GDGCH/C    
BspQI § GCTCTTC(1/4)    
BsrBI § CCGCTC(-3/-3)    
BsrDI § GCAATG(2/0)    
BsrFI-v2 § R/CCGGY    
BsrGI § T/GTACA    
BsrGI-HF® § T/GTACA    
BsrI § ACTGG(1/-1)  
BssHII § G/CGCGC    
BssSI-v2 § CACGAG(-5/-1)    
BstBI § TT/CGAA    
BstEII  § G/GTNACC    
BstEII-HF® § G/GTNACC    
BstNI § CC/WGG    
BstUI § CG/CG  
BstXI § CCANNNNN/NTGG    
BstYI § R/GATCY    
BstZ17I-HF® § GTATAC    
Bsu36I § CC/TNAGG    
BtgI § C/CRYGG    
BtsCI § GGATG(2/0)    
BtsI-v2 § GCAGTG(2/0)    
Cac8I § GCN/NGC  
ClaI § AT/CGAT    
CspCI § (11/13)CAANNNNNGTGG(12/10)    
CviAII § C/ATG    
CviQI § G/TAC    
DdeI § C/TNAG    
DpnI § GA/TC    
DpnII § /GATC    
DraI § TTT/AAA    
DraIII-HF® § CACNNN/GTG    
DrdI § GACNNNN/NNGTC    
EagI-HF® § C/GGCCG    
EarI § CTCTTC(1/4)    
Eco53kI § GAG/CTC    
EcoNI § CCTNN/NNNAGG    
EcoO109I § RG/GNCCY    
EcoP15I § CAGCAG(25/27)    
EcoRI § G/AATTC    
EcoRI-HF® § G/AATTC    
EcoRV § GAT/ATC    
EcoRV-HF® § GAT/ATC    
Esp3I § CGTCTC(1/5)    
Fnu4HI § GC/NGC    
FseI § GGCCGG/CC    
FspI § TGC/GCA    
HaeII § RGCGC/Y    
HaeIII § GG/CC    
HhaI § GCG/C    
HincII § GTY/RAC    
HindIII-HF® § A/AGCTT    
HinfI § G/ANTC    
HinP1I § G/CGC    
HpaII § C/CGG    
HphI § GGTGA(8/7)    
HpyAV § CCTTC(6/5)     NT
HpyCH4IV § A/CGT    
HpyCH4V § TG/CA    
Hpy166II § GTN/NAC    
KpnI-HF® § GGTAC/C    
MboI § /GATC    
MboII § GAAGA(8/7)    
MfeI-HF® § C/AATTG    
MluCI § /AATT    
MluI § A/CGCGT    
MluI-HF® § A/CGCGT    
MlyI § GAGTC(5/5)    
MmeI § TCCRAC(20/18)    
MnlI § CCTC(7/6)    
MseI § T/TAA    
MslI § CAYNN/NNRTG    
MspA1I § CMG/CKG    
MspI § C/CGG    
MwoI § GCNNNNN/NNGC    
NciI § CC/SGG    
NcoI § C/CATGG    
NcoI-HF® § C/CATGG    
NdeI § CA/TATG    
NgoMIV § G/CCGGC    
NheI-HF® § G/CTAGC    
NlaIII § CATG/    
NotI § GC/GGCCGC    
NotI-HF® § GC/GGCCGC    
NruI § TCG/CGA    
NruI-HF® § TCG/CGA    
NsiI § ATGCA/T    
NsiI-HF® § ATGCA/T    
NspI § RCATG/Y    
PacI § TTAAT/TAA    
PaeR7I § C/TCGAG    
PflFI § GACN/NNGTC    
PflMI § CCANNNN/NTGG    
PmeI § GTTT/AAAC     NT
PmlI § CAC/GTG    
PpuMI § RG/GWCCY    
PshAI § GACNN/NNGTC    
PsiI-v2 § TTA/TAA    
PstI § CTGCA/G    
PstI-HF® § CTGCA/G    
PvuI § CGAT/CG    
PvuI-HF® § CGAT/CG    
PvuII § CAG/CTG    
PvuII-HF® § CAG/CTG    
RsaI § GT/AC    
SacI § GAGCT/C    
SacI-HF® § GAGCT/C    
SacII § CCGC/GG    
SalI § G/TCGAC    
SalI-HF® § G/TCGAC    
SapI § GCTCTTC(1/4)    
SbfI § CCTGCA/GG    
SbfI-HF® § CCTGCA/GG    
ScaI-HF® § AGT/ACT    
SfiI § GGCCNNNN/NGGCC    
SfoI § GGC/GCC    
SmaI § CCC/GGG    
SpeI § A/CTAGT    
SpeI-HF® § A/CTAGT    
SphI-HF® § GCATG/C    
SrfI § GCCC/GGGC    
SspI § AAT/ATT    
SspI-HF® § AAT/ATT    
StuI § AGG/CCT    
StyD4I § /CCNGG    
StyI-HF® § C/CWWGG    
SwaI § ATTT/AAAT    
TaqI-v2 § T/CGA    
TfiI § G/AWTC    
TseI § G/CWGC    
TspMI § C/CCGGG  
TspRI § NNCASTGNN/    
Tth111I § GACN/NNGTC    
XbaI § T/CTAGA    
XhoI § C/TCGAG    
XmaI § C/CCGGG    
XmnI § GAANN/NNTTC    

§ An HF version of this enzyme is available.

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