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Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

Note: These diluents are not for use with Thermophilic DNA Polymerases.

Diluent Buffer A
Acc65I AccI AciI AflII AgeI-HF®
AhdI AlwI AlwNI ApaI ApaLI
ApoI§ AscI AvaI AvaII BaeGI
BaeI BamHI§ BamHI-HF® BanI BanII
BccI BceAI BcgI BclI§ BglII
BlpI BsaJI BsaWI BseRI BsiEI
BsiHKAI BslI BsmFI BsmI BsoBI
Bsp1286I BspCNI BspDI BspHI BsrBI
BsrDI BsrGI§ BsrGI-HF® BstAPI BstBI
BstEII § BstEII-HF® BstNI BstUI BstYI
BstZ17I-HF® BtgZI BtsIMutI BtsI-v2 ClaI
CspCI CviKI-1 DraI DrdI EaeI
EciI Eco53kI EcoNI EcoO109I EcoP15I
EcoRV§ FatI FauI Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinfI
HinP1I HpaI HpaII Hpy188I Hpy99I
HpyCH4III HpyCH4IV HpyCH4V KpnI-HF® MboI
MfeI-HF® MluCI MluI§ MluI-HF® MlyI
MseI MslI MspI NaeI NarI
Nb.BbvCI Nb.BsmI Nb.BsrDI Nb.BtsI NciI
NcoI§ NdeI NgoMIV NotI-HF® NruI§
NruI-HF® NspI Nt.AlwI Nt.BbvCI Nt.BsmAI
Nt.BstNBI Nt.CviPII PacI PaeR7I PflFI
PflMI PleI PluTI PmeI PmlI
PshAI PspGI RsaI SacI-HF® SacII
SalI§ SalI-HF® Sau3AI Sau96I SexAI
SgrAI SmlI SnaBI StuI StyI-HF®
Tsp45I WarmStart® Nt.BstNBI XbaI XhoI XmaI
XmnI
Diluent Buffer B
AatII AclI AcuI AfeI AflIII
AleI-v2 AluI ApeKI ApoI-HF AseI
AsiSI AvrII BbsI§ BbsI-HF® BbvCI
BbvI BclI-HF BcoDI BfaI BfuAI
BglI BmgBI BmrI BmtI§ BmtI-HF®
BpmI Bpu10I BpuEI BsaBI BsaI-HF®v2
BseYI BsgI BsiWI§ BsiWI-HF® BsmAI
BsmBI-v2 BspEI BspMI BspQI BsrI
BssHII BssSI-v2 BstXI BtgI BtsCI
Cac8I DdeI DpnI DpnII DraIII-HF®
EagI-HF® EarI EcoRV-HF® Esp3I FseI
FspEI HincII HindIII§ HindIII-HF® HphI
Hpy188III I-CeuI I-SceI KasI LpnPI
MmeI MnlI MspA1I MspJI MwoI
Nb.BssSI NcoI-HF® NlaIII NlaIV NmeAIII
NsiI§ NsiI-HF® Nt.BspQI PaqCI PciI
PI-PspI PI-SceI PpuMI PsiI-v2 PspOMI
PspXI PvuI§ PvuI-HF® PvuII§ PvuII-HF®
SapI SbfI-HF® ScaI-HF® SfaNI SfcI
SfoI SmaI SphI§ SphI-HF® SrfI
SspI-HF® StyD4I SwaI TaqI-v2 TseI
TspMI TspRI Tth111I ZraI
Diluent Buffer C
AbaSI BciVI BsaAI BsaHI BsaXI
BsrFI-v2 Bsu36I CviAII CviQI EcoRI§
EcoRI-HF® FspI Hpy166II MboII MscI
NheI-HF® NotI§ PstI§ PstI-HF® RsrII
ScrFI SfiI SpeI-HF® SspI§ TfiI
XcmI

§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.

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