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Home Tools & Resources Usage Guidelines Restriction Enzyme Diluent Buffer Compatibility

Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

Note: These diluents are not for use with Thermophilic DNA Polymerases.

Diluent Buffer A
Acc65I AccI AciI AflII AgeI-HF®
AhdI AlwI AlwNI ApaI ApaLI
AscI AvaI AvaII BaeGI BaeI
BamHI§ BamHI-HF® BanI BanII BccI
BceAI BcgI BclI§ BglII BlpI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BslI BsmFI BsmI BsoBI Bsp1286I
BspCNI BspDI BspHI BsrBI BsrDI
BsrGI-HF® BssKI BstAPI BstBI BstEII-HF®
BstNI BstUI BstYI BstZ17I-HF® BtgZI
BtsI BtsIMutI BtsI-v2 ClaI CspCI
CviKI-1 DraI DrdI EaeI EciI
Eco53kI EcoNI EcoO109I EcoP15I EcoRV§
FatI FauI Fnu4HI FokI HaeII
HaeIII HgaI HhaI HinfI HinP1I
HpaI HpaII Hpy188I Hpy99I HpyCH4III
HpyCH4IV HpyCH4V KpnI-HF® MboI MfeI-HF®
MluCI MluI-HF® MlyI MseI MslI
MspI NaeI NarI Nb.BbvCI Nb.BsmI
Nb.BsrDI Nb.BtsI NciI NcoI§ NdeI
NgoMIV NotI-HF® NruI-HF® NspI Nt.AlwI
Nt.BbvCI Nt.BsmAI Nt.BstNBI Nt.CviPII PacI
PaeR7I PflFI PflMI PhoI PleI
PluTI PmeI PmlI PshAI PspGI
RsaI SacI-HF® SacII SalI§ SalI-HF®
Sau3AI Sau96I ScaI§ SexAI SgrAI
SmlI SnaBI StuI StyI-HF® Tsp45I
Tsp509I WarmStart® Nt.BstNBI XbaI XhoI XmaI
XmnI
Diluent Buffer B
AatII AclI AcuI AfeI AflIII
AleI AleI-v2 AluI ApeKI ApoI-HF
AseI AsiSI AvrII BbsI§ BbsI-HF®
BbvCI BbvI BclI-HF BcoDI BfaI
BfuAI BfuCI BglI BmgBI BmrI
BmtI-HF® BpmI Bpu10I BpuEI BsaBI
BsaI-HF® BsaI-HF®v2 BseYI BsgI BsiWI§
BsiWI-HF® BsmAI BsmBI-v2 BspEI BspMI
BspQI BsrI BssHII BssSI BssSI-v2
BstXI BstZ17I§ BtgI BtsCI Cac8I
DdeI DpnI DpnII DraIII§ DraIII-HF®
EagI-HF® EarI EcoRV-HF® Esp3I FseI
FspEI HincII HindIII§ HindIII-HF® HphI
Hpy188III I-CeuI I-SceI KasI LpnPI
MmeI MnlI MspA1I MspJI MwoI
Nb.BssSI NcoI-HF® NlaIII NlaIV NmeAIII
NsiI§ NsiI-HF® Nt.BspQI PaqCI PciI
PI-PspI PI-SceI PpuMI PsiI PsiI-v2
PspOMI PspXI PvuI-HF® PvuII§ PvuII-HF®
SapI SbfI-HF® ScaI-HF® SfaNI SfcI
SfoI SmaI SphI§ SphI-HF® SrfI
SspI-HF® StyD4I SwaI TaqI-v2 TliI
TseI TspMI TspRI Tth111I ZraI
Diluent Buffer C
AbaSI BciVI BsaAI BsaHI BsaXI
BsrFI BsrFI-v2 Bsu36I CviAII CviQI
EcoRI§ EcoRI-HF® FspI Hpy166II MboII
MscI NheI-HF® NotI§ PstI§ PstI-HF®
RsrII ScrFI SfiI SpeI-HF® TfiI
XcmI

§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.

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