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Home Tools & Resources Usage Guidelines Restriction Enzyme Diluent Buffer Compatibility

Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

Note: These diluents are not for use with Thermophilic DNA Polymerases.

Diluent Buffer A
Acc65I AccI AciI AflII AgeI-HF®
AhdI AlwI AlwNI ApaI ApaLI
AscI AvaI AvaII BaeGI BaeI
BamHI§ BamHI-HF® BanI BanII BccI
BceAI BcgI BclI§ BglII BlpI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BslI BsmFI BsmI BsoBI Bsp1286I
BspCNI BspDI BspHI BsrBI BsrDI
BsrGI-HF® BstAPI BstBI BstEII-HF® BstNI
BstUI BstYI BstZ17I-HF® BtgZI BtsIMutI
BtsI-v2 ClaI CspCI CviKI-1 DraI
DrdI EaeI EciI Eco53kI EcoNI
EcoO109I EcoP15I EcoRV§ FatI FauI
Fnu4HI FokI HaeII HaeIII HgaI
HhaI HinfI HinP1I HpaI HpaII
Hpy188I Hpy99I HpyCH4III HpyCH4IV HpyCH4V
KpnI-HF® MboI MfeI-HF® MluCI MluI-HF®
MlyI MseI MslI MspI NaeI
NarI Nb.BbvCI Nb.BsmI Nb.BsrDI Nb.BtsI
NciI NcoI§ NdeI NgoMIV NotI-HF®
NruI-HF® NspI Nt.AlwI Nt.BbvCI Nt.BsmAI
Nt.BstNBI Nt.CviPII PacI PaeR7I PflFI
PflMI PleI PluTI PmeI PmlI
PshAI PspGI RsaI SacI-HF® SacII
SalI§ SalI-HF® Sau3AI Sau96I SexAI
SgrAI SmlI SnaBI StuI StyI-HF®
Tsp45I WarmStart® Nt.BstNBI XbaI XhoI XmaI
XmnI
Diluent Buffer B
AatII AclI AcuI AfeI AflIII
AleI-v2 AluI ApeKI ApoI-HF AseI
AsiSI AvrII BbsI§ BbsI-HF® BbvCI
BbvI BclI-HF BcoDI BfaI BfuAI
BglI BmgBI BmrI BmtI-HF® BpmI
Bpu10I BpuEI BsaBI BsaI-HF®v2 BseYI
BsgI BsiWI§ BsiWI-HF® BsmAI BsmBI-v2
BspEI BspMI BspQI BsrI BssHII
BssSI-v2 BstXI BtgI BtsCI Cac8I
DdeI DpnI DpnII DraIII-HF® EagI-HF®
EarI EcoRV-HF® Esp3I FseI FspEI
HincII HindIII§ HindIII-HF® HphI Hpy188III
I-CeuI I-SceI KasI LpnPI MmeI
MnlI MspA1I MspJI MwoI Nb.BssSI
NcoI-HF® NlaIII NlaIV NmeAIII NsiI§
NsiI-HF® Nt.BspQI PaqCI PciI PI-PspI
PI-SceI PpuMI PsiI-v2 PspOMI PspXI
PvuI-HF® PvuII§ PvuII-HF® SapI SbfI-HF®
ScaI-HF® SfaNI SfcI SfoI SmaI
SphI§ SphI-HF® SrfI SspI-HF® StyD4I
SwaI TaqI-v2 TseI TspMI TspRI
Tth111I ZraI
Diluent Buffer C
AbaSI BciVI BsaAI BsaHI BsaXI
BsrFI-v2 Bsu36I CviAII CviQI EcoRI§
EcoRI-HF® FspI Hpy166II MboII MscI
NheI-HF® NotI§ PstI§ PstI-HF® RsrII
ScrFI SfiI SpeI-HF® TfiI XcmI

§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.

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