I have been asked “What is overcycling and how can I avoid it?”
Overcycling happened during the PCR when the reaction has run out of PCR primer. You will see large molecular weight fragments appear on the bioanalyzer. If you think that you have overcycling issues, you can just try to reduce the number of PCR cycles next time.
Remember, you can always get additional technical support by contacting info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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