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Restriction Endonucleases

Cut Smarter with Restriction Enzymes from NEB

With over 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

Convenience

  • A vial of 6X Purple Load Dye is included with most restriction enzymes.
  • Over 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. 
  • >190 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
  • Choose from >275 restriction enzymes, the largest selection commercially available.



Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.


Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.


Choose Product:

Restriction Endonucleases

Restriction Endonucleases: A

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI-HF®

AhdI

AleI-v2

AluI

AlwI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

Restriction Endonucleases: B

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BsaAI

BsaBI

BsaHI

BsaI-HF®v2

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI-v2

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI-v2

BsrGI

BsrGI-HF®

BsrI

BssHII

BssSI-v2

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I-HF®

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsI-v2

Restriction Endonucleases: C-G

Cac8I

ClaI

CspCI

CviAII

CviKI-1

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

Esp3I

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

Restriction Endonucleases: H-M

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188I

Hpy188III

Hpy99I

HpyAV

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI-HF®

LpnPI

MboI

MboII

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

Restriction Endonucleases: N-O

NaeI

NarI

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Nt.CviPII

Restriction Endonucleases: P-R

PacI

PaeR7I

PaqCI®

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PluTI

PmeI

PmlI

PpuMI

PshAI

PsiI-v2

PspGI

PspOMI

PspXI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

RsrII

Restriction Endonucleases: S

SacI-HF®

SacII

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI-HF®

SwaI

Restriction Endonucleases: T-Z

TaqI-v2

TfiI

TseI

Tsp45I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI

HF®, Nicking, Master Mix, Time-Saver & Other

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AflII

AgeI-HF®

AhdI

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BbsI

BbsI-HF®

BbvI

BciVI

BclI

BclI-HF

BcoDI

BfuAI

BglI

BglII

BlpI

BmgBI

BmtI-HF®

BpmI

BpuEI

BsaAI

BsaHI

BsaI-HF®v2

BsaWI

BsaXI

BseRI

BsgI

BsiEI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI-v2

BsmI

BsoBI

Bsp1286I

BspCNI

BspEI

BspHI

BspQI

BsrBI

BsrDI

BsrGI

BsrGI-HF®

BsrI

BssHII

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I-HF®

Bsu36I

BtgI

BtsCI

Cac8I

ClaI

CspCI

CviAII

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EagI-HF®

EarI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

Esp3I

Fnu4HI

FseI

FspEI

FspI

HaeIII

HhaI

HincII

HindIII-HF®

HinfI

HinP1I

HpaII

HphI

Hpy166II

HpyAV

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KpnI-HF®

LpnPI

MboI

McrBC

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI-HF®

NlaIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Nt.CviPII

PacI

PaeR7I

PflFI

PflMI

PI-PspI

PI-SceI

PmeI

PmlI

PpuMI

PshAI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

SbfI-HF®

ScaI-HF®

SfiI

SfoI

SmaI

SpeI-HF®

SphI-HF®

SspI-HF®

StuI

StyD4I

StyI-HF®

SwaI

TaqI-v2

TfiI

TseI

TspMI

TspRI

Tth111I

XbaI

XhoI

XmaI

XmnI

Restriction Endonuclease Buffers & Diluents

BSA, Molecular Biology Grade

Diluent A

Diluent B

Diluent C

NEBuffer™ 1

NEBuffer™ 2

NEBuffer™ 3

NEBuffer™ 4

NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)

rCutSmart™ Buffer

Recombinant Albumin, Molecular Biology Grade

S-adenosylmethionine (SAM)

Restriction Endonucleases: A

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI-HF®

AhdI

AleI-v2

AluI

AlwI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

Restriction Endonucleases: B

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BsaAI

BsaBI

BsaHI

BsaI-HF®v2

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI-v2

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI-v2

BsrGI

BsrGI-HF®

BsrI

BssHII

BssSI-v2

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I-HF®

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsI-v2

Restriction Endonucleases: C-G

Cac8I

ClaI

CspCI

CviAII

CviKI-1

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

Esp3I

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

Restriction Endonucleases: H-M

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188I

Hpy188III

Hpy99I

HpyAV

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI-HF®

LpnPI

MboI

MboII

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

Restriction Endonucleases: N-O

NaeI

NarI

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Nt.CviPII

Restriction Endonucleases: P-R

PacI

PaeR7I

PaqCI®

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PluTI

PmeI

PmlI

PpuMI

PshAI

PsiI-v2

PspGI

PspOMI

PspXI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

RsrII

Restriction Endonucleases: S

SacI-HF®

SacII

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI-HF®

SwaI

Restriction Endonucleases: T-Z

TaqI-v2

TfiI

TseI

Tsp45I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI

HF®, Nicking, Master Mix, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

AgeI-HF®

ApoI-HF®

BamHI-HF®

BbsI-HF®

BclI-HF

BmtI-HF®

BsaI-HF®v2

BsiWI-HF®

BsrGI-HF®

BstEII-HF®

BstZ17I-HF®

DraIII-HF®

EagI-HF®

EcoRI-HF®

EcoRV-HF®

HindIII-HF®

KpnI-HF®

MfeI-HF®

MluI-HF®

NcoI-HF®

NheI-HF®

NotI-HF®

NruI-HF®

NsiI-HF®

PstI-HF®

PvuI-HF®

PvuII-HF®

SacI-HF®

SalI-HF®

SbfI-HF®

ScaI-HF®

SpeI-HF®

SphI-HF®

SspI-HF®

StyI-HF®

Homing Endonucleases

I-CeuI

I-SceI

PI-PspI

PI-SceI

Nicking Endonucleases

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Nt.CviPII

Restriction Enzymes for Epigenetic Analysis

AbaSI

DpnI

DpnII

FspEI

HpaII

LpnPI

McrBC

MspI

MspJI

Time-Saver™ Qualified Restriction Enzymes

AatII

Acc65I

AccI

AciI

AclI

AcuI

AflII

AgeI-HF®

AhdI

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI

ApoI-HF®

AscI

AseI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BbsI

BbsI-HF®

BbvI

BciVI

BclI

BclI-HF

BcoDI

BfuAI

BglI

BglII

BlpI

BmgBI

BmtI-HF®

BpmI

BpuEI

BsaAI

BsaHI

BsaI-HF®v2

BsaWI

BsaXI

BseRI

BsgI

BsiEI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI-v2

BsmI

BsoBI

Bsp1286I

BspCNI

BspEI

BspHI

BspQI

BsrBI

BsrDI

BsrGI

BsrI

BssHII

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I-HF®

Bsu36I

BtgI

BtsCI

Cac8I

ClaI

CspCI

CviAII

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EagI-HF®

EarI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

Esp3I

Fnu4HI

FseI

FspI

HaeIII

HhaI

HincII

HindIII-HF®

HinfI

HinP1I

HpaII

HphI

Hpy166II

HpyAV

HpyCH4IV

HpyCH4V

KpnI-HF®

MboI

MfeI-HF®

MluCI

MluI

MlyI

MmeI

MnlI

MseI

MslI

MspA1I

MspI

MwoI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI-HF®

NlaIII

NotI

NotI-HF®

NruI

NsiI

NspI

PacI

PaeR7I

PflFI

PflMI

PmeI

PmlI

PpuMI

PshAI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

SbfI-HF®

ScaI-HF®

SfiI

SfoI

SmaI

SpeI-HF®

SphI-HF®

SspI-HF®

StuI

StyD4I

StyI-HF®

SwaI

TaqI-v2

TfiI

TseI

TspMI

TspRI

Tth111I

XbaI

XhoI

XmaI

XmnI

Restriction Endonuclease Buffers & Diluents

BSA, Molecular Biology Grade

Diluent A

Diluent B

Diluent C

NEBuffer™ 1

NEBuffer™ 2

NEBuffer™ 3

NEBuffer™ 4

NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)

rCutSmart™ Buffer

Recombinant Albumin, Molecular Biology Grade

S-adenosylmethionine (SAM)

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